TEV protease Recombinant Protein

Product Specifications

Overview

Purified recombinant protein of TEV protease, with N-terminal HIS tag, expressed in E.Coli

Expression System

E. coli

Tag

N-His

Concentration

>50 ug/mL as determined by Bradford protein assay method.

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Activity

1 unit is defined as the amount of enzyme required to cleave 3 µg of a substrate protein to 85% completion in 1 hours at 30°C.

Form

Liquid

Buffer

25mM Tris, 150mM NaCl, 1mM EDTA, 5mM DTT, 50% glycerol, pH8.0

Molecular Weight

29.0 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.