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Gfer (NM_023040) Mouse Recombinant Protein

Product Specifications

Background

FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse growth factor, augmenter of liver regeneration (Gfer), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

Alr; ERV1

Gene ID

11692

UniProt

P56213

Accession Number mRNA

NM_023040

Chromosomal Location

17 12.48 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

22.9 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

Gfer

Species

Mouse

Protein ID

NP_075527

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/11692

Uniprot URL

https://www.uniprot.org/uniprot/P56213

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_023040

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_075527

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