What is the catalog number for DHFR (NM_000791) Human Recombinant Protein?

The catalog number for DHFR (NM_000791) Human Recombinant Protein is PH42242M5.

What sizes are available for DHFR (NM_000791) Human Recombinant Protein?

DHFR (NM_000791) Human Recombinant Protein is available in the following size(s): 20 µg.

Is DHFR (NM_000791) Human Recombinant Protein in stock?

DHFR (NM_000791) Human Recombinant Protein is currently made to order / not held in immediate stock. Contact us for lead time.

DHFR (NM_000791) Human Recombinant Protein

Product Specifications

Background

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]

Overview

Recombinant protein of human dihydrofolate reductase (DHFR)

Synonyms

DHFRP1; DYR

Gene ID

1719

UniProt

P00374, B0YJ76

Accession Number mRNA

NM_000791

Chromosomal Location

5q14.1

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Folate biosynthesis, Metabolic pathways, One carbon pool by folate

Field of Research

Folate biosynthesis, Metabolic pathways, One carbon pool by folate

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome, Stem cell - Pluripotency

Molecular Weight

21.3 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.