DARS1 (NM_001349) Human Recombinant Protein

Product Specifications

Background

This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA (Asp) . Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Overview

Recombinant protein of human aspartyl-tRNA synthetase (DARS)

Synonyms

AspRS; DARS; HBSL

Gene ID

1615

UniProt

P14868, A0A140VJW5

Accession Number mRNA

NM_001349

Chromosomal Location

2q21.3

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Aminoacyl-tRNA biosynthesis

Field of Research

Aminoacyl-tRNA biosynthesis

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Molecular Weight

57 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.