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HPRT (HPRT1) (NM_000194) Human Recombinant Protein

Product Specifications

Background

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]

Synonyms

HGPRT; HPRT

Gene ID

3251

UniProt

P00492, A0A140VJL3

Accession Number mRNA

NM_000194

Chromosomal Location

Xq26.2-q26.3

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism

Field of Research

Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome, Stem cell - Pluripotency

Molecular Weight

24.4 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

HPRT1

Species

Human

Protein ID

NP_000185

Overview

Recombinant protein of human hypoxanthine phosphoribosyltransferase 1 (HPRT1)

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/3251

Uniprot URL

https://www.uniprot.org/uniprot/P00492

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_000194

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_000185

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