CDC25A (NM_001789) Human Recombinant Protein

Product Specifications

Background

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Overview

Recombinant protein of human cell division cycle 25 homolog A (S. pombe) (CDC25A), transcript variant 1

Synonyms

CDC25A2

Gene ID

993

UniProt

P30304

Accession Number mRNA

NM_001789

Chromosomal Location

3p21.31

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Cell cycle, Progesterone-mediated oocyte maturation

Field of Research

Cell cycle, Progesterone-mediated oocyte maturation

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome, Phosphatase

Molecular Weight

58.9 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.