DDOST (NM_005216) Human Recombinant Protein

Product Specifications

Background

This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008]

Overview

Recombinant protein of human dolichyl-diphosphooligosaccharide-protein glycosyltransferase (DDOST)

Synonyms

AGER1; CDG1R; GATD6; OKSWcl45; OST; OST48; WBP1

Gene ID

1650

UniProt

P39656, A0A024RAD5

Accession Number mRNA

NM_005216

Chromosomal Location

1p36.12

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Metabolic pathways, N-Glycan biosynthesis

Field of Research

Metabolic pathways, N-Glycan biosynthesis

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Transmembrane

Molecular Weight

46.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.