RFC2 (NM_002914) Human Recombinant Protein

Product Specifications

Background

This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2. [provided by RefSeq, Jul 2013]

Overview

Recombinant protein of human replication factor C (activator 1) 2, 40kDa (RFC2), transcript variant 2

Synonyms

RFC40

Gene ID

5982

UniProt

P35250, Q75MT5

Accession Number mRNA

NM_002914

Chromosomal Location

7q11.23

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

DNA replication, Mismatch repair, Nucleotide excision repair

Field of Research

DNA replication, Mismatch repair, Nucleotide excision repair

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome, Stem cell - Pluripotency

Molecular Weight

35.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.