Phosphoribosyl pyrophosphate amidotransferase (PPAT) (NM_002703) Human Recombinant Protein

Product Specifications

Background

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011]

Overview

Recombinant protein of human phosphoribosyl pyrophosphate amidotransferase (PPAT)

Synonyms

ATASE; GPAT; PRAT

Gene ID

5471

UniProt

Q06203, A8K4H7, Q59G63

Accession Number mRNA

NM_002703

Chromosomal Location

4q12

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Alanine, aspartate and glutamate metabolism, Metabolic pathways, Purine metabolism

Field of Research

Alanine, aspartate and glutamate metabolism, Metabolic pathways, Purine metabolism

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome, Protease

Molecular Weight

57.2 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.