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ATP6V1E1 (NM_001696) Human Recombinant Protein

Product Specifications

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq, Jul 2008]

Synonyms

ARCL2C; ATP6E; ATP6E2; ATP6V1E; P31; Vma4

Gene ID

529

UniProt

P36543, Q53Y06

Accession Number mRNA

NM_001696

Chromosomal Location

22q11.21

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection

Field of Research

Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Molecular Weight

26 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

ATP6V1E1

Species

Human

Protein ID

NP_001687

Overview

Recombinant protein of human ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 (ATP6V1E1), transcript variant 1

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/529

Uniprot URL

https://www.uniprot.org/uniprot/P36543

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_001696

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_001687

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