DOHH (NM_031304) Human Recombinant Protein
Product Specifications
Background
This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2009]
Synonyms
HDOHH; HLRC1
Gene ID
83475
UniProt
Q9BU89
Accession Number mRNA
NM_031304
Chromosomal Location
19p13.3
Expression System
HEK293T
Tag
C-Myc/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Molecular Weight
32.7 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
DOHH
Species
Human
Protein ID
NP_112594
Overview
Recombinant protein of human deoxyhypusine hydroxylase/monooxygenase (DOHH), transcript variant 2
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/83475
Uniprot URL
https://www.uniprot.org/uniprot/Q9BU89
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_031304
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_112594
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