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MRPS25 (NM_022497) Human Recombinant Protein

Product Specifications

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Synonyms

COXPD50; MRP-S25; RPMS25

Gene ID

64432

UniProt

P82663

Accession Number mRNA

NM_022497

Chromosomal Location

3p25.1

Expression System

HEK293T

Tag

C-Myc/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Molecular Weight

19.9 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

MRPS25

Species

Human

Protein ID

NP_071942

Overview

Recombinant protein of human mitochondrial ribosomal protein S25 (MRPS25), nuclear gene encoding mitochondrial protein

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/64432

Uniprot URL

https://www.uniprot.org/uniprot/P82663

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_022497

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_071942

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