MRPS25 (NM_022497) Human Recombinant Protein
Product Specifications
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Synonyms
COXPD50; MRP-S25; RPMS25
Gene ID
64432
UniProt
P82663
Accession Number mRNA
NM_022497
Chromosomal Location
3p25.1
Expression System
HEK293T
Tag
C-Myc/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Molecular Weight
19.9 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
MRPS25
Species
Human
Protein ID
NP_071942
Overview
Recombinant protein of human mitochondrial ribosomal protein S25 (MRPS25), nuclear gene encoding mitochondrial protein
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/64432
Uniprot URL
https://www.uniprot.org/uniprot/P82663
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_022497
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_071942
Curated Selection
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