MLH1 (NM_000249) Human Recombinant Protein

Product Specifications

Background

The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) . [provided by RefSeq, Aug 2017]

Overview

Recombinant protein of human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1)

Synonyms

COCA2; FCC2; hMLH1; HNPCC; HNPCC2; MMRCS1

Gene ID

4292

UniProt

P40692

Accession Number mRNA

NM_000249

Chromosomal Location

3p22.2

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer

Field of Research

Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome

Molecular Weight

84.4 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.