Aspartate Aminotransferase (GOT1) (NM_002079) Human Recombinant Protein
Product Specifications
Background
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]
Overview
Recombinant protein of human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1)
Synonyms
AST1; ASTQTL1; cAspAT; cCAT; GIG18
Gene ID
2805
UniProt
P17174, A0A140VK69
Accession Number mRNA
NM_002079
Chromosomal Location
10q24.2
Expression System
HEK293T
Tag
C-Myc/DDK
Related Pathways
Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism
Field of Research
Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Molecular Weight
46.1 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
GOT1
Species
Human
Protein ID
NP_002070
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/2805
Uniprot URL
https://www.uniprot.org/uniprot/P17174
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_002079
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_002070
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