U1A (SNRPA) (NM_004596) Human Recombinant Protein

Product Specifications

Background

The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation. [provided by RefSeq, Oct 2010]

Overview

Recombinant protein of human small nuclear ribonucleoprotein polypeptide A (SNRPA)

Synonyms

Mud1; U1-A; U1A

Gene ID

6626

UniProt

P09012

Accession Number mRNA

NM_004596

Chromosomal Location

19q13.2

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Spliceosome

Field of Research

Spliceosome

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Activity

Leading Biology human recombinant small nuclear ribonucleoprotein polypeptide A (SNRPA) was compared side-by-side with baculovirus based insect cell (BEVS) derived SNRPA in a phycoerythrin detecting Luminex assay. The human cell produced SNRPA is comparative or better in sensitivity than the insect cell produced SNRPA to detect autoantibodies in human serum samples.

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Stem cell - Pluripotency

Molecular Weight

31.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.