14 3 3 gamma (YWHAG) (NM_012479) Human Recombinant Protein

Product Specifications

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]

Overview

Recombinant protein of human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (YWHAG)

Synonyms

14-3-3GAMMA; DEE56; EIEE56; PPP1R170

Gene ID

7532

UniProt

P61981

Accession Number mRNA

NM_012479

Chromosomal Location

7q11.23

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis

Field of Research

Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome

Molecular Weight

28.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.