UBE2H (NM_003344) Human Recombinant Protein
Product Specifications
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011]
Synonyms
E2-20K; GID3; UBC8; UBCH; UBCH2
Gene ID
7328
UniProt
P62256, A4D1L5
Accession Number mRNA
NM_003344
Chromosomal Location
7q32.2
Expression System
HEK293T
Tag
C-Myc/DDK
Related Pathways
Ubiquitin mediated proteolysis
Field of Research
Ubiquitin mediated proteolysis
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Molecular Weight
20.5 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
UBE2H
Species
Human
Protein ID
NP_003335
Overview
Recombinant protein of human ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) (UBE2H), transcript variant 1
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/7328
Uniprot URL
https://www.uniprot.org/uniprot/P62256
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_003344
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_003335
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