NFYB (NM_006166) Human Recombinant Protein

Product Specifications

Background

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]

Overview

Recombinant protein of human nuclear transcription factor Y, beta (NFYB)

Synonyms

CBF-A; CBF-B; HAP3; NF-YB

Gene ID

4801

UniProt

P25208, A0A024RBG7

Accession Number mRNA

NM_006166

Chromosomal Location

12q23.3

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Antigen processing and presentation

Field of Research

Antigen processing and presentation

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Transcription Factors

Molecular Weight

22.7 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.