NFYB (NM_006166) Human Recombinant Protein
Product Specifications
Background
The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]
Synonyms
CBF-A; CBF-B; HAP3; NF-YB
Gene ID
4801
UniProt
P25208, A0A024RBG7
Accession Number mRNA
NM_006166
Chromosomal Location
12q23.3
Expression System
HEK293T
Tag
C-Myc/DDK
Related Pathways
Antigen processing and presentation
Field of Research
Antigen processing and presentation
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Function
Transcription Factors
Molecular Weight
22.7 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
NFYB
Species
Human
Protein ID
NP_006157
Overview
Recombinant protein of human nuclear transcription factor Y, beta (NFYB)
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/4801
Uniprot URL
https://www.uniprot.org/uniprot/P25208
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_006166
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_006157
Curated Selection
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