DAP13 (NDUFA12) (NM_018838) Human Recombinant Protein
Product Specifications
Background
This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]
Overview
Recombinant protein of human NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12 (NDUFA12)
Synonyms
B17.2; DAP13; MC1DN23
Gene ID
55967
UniProt
Q9UI09
Accession Number mRNA
NM_018838
Chromosomal Location
12q22
Expression System
HEK293T
Tag
C-Myc/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Molecular Weight
16.9 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
NDUFA12
Species
Human
Protein ID
NP_061326
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/55967
Uniprot URL
https://www.uniprot.org/uniprot/Q9UI09
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_018838
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_061326
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