PCIF1 (NM_022104) Human Recombinant Protein

Product Specifications

Background

Cap-specific adenosine methyltransferase that catalyzes formation of N (6),2'-O-dimethyladenosine cap (m6A (m) ) by methylating the adenosine at the second transcribed position of capped mRNAs (PubMed:30467178) . Recruited to the early elongation complex of RNA polymerase II (RNAPII) via interaction with POLR2A and mediates formation of m6A (m) co-transcriptionally (PubMed:30467178) . N6-methylation of m6A (m) promotes the translation of capped mRNAs (PubMed:30467178) .[UniProtKB/Swiss-Prot Function]

Overview

Recombinant protein of human PDX1 C-terminal inhibiting factor 1 (PCIF1)

Synonyms

C20orf67; CAPAM; hCAPAM; hPCIF1; PPP1R121

Gene ID

63935

UniProt

Q9H4Z3

Accession Number mRNA

NM_022104

Chromosomal Location

20q13.12

Expression System

HEK293T

Tag

C-Myc/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Molecular Weight

80.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.