Alanine Transaminase (GPT) (NM_005309) Human Recombinant Protein
Product Specifications
Background
This gene encodes cytosolic alanine aminotransaminase 1 (ALT1) ; also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]
Synonyms
AAT1; ALT1; GPT1
Gene ID
2875
UniProt
P24298
Accession Number mRNA
NM_005309
Chromosomal Location
8q24.3
Expression System
HEK293T
Tag
C-Myc/DDK
Related Pathways
Alanine, aspartate and glutamate metabolism, Metabolic pathways
Field of Research
Alanine, aspartate and glutamate metabolism, Metabolic pathways
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Function
Druggable Genome
Molecular Weight
54.5 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
GPT
Species
Human
Protein ID
NP_005300
Overview
Recombinant protein of human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT)
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/2875
Uniprot URL
https://www.uniprot.org/uniprot/P24298
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_005309
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_005300
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