Alanine Transaminase (GPT) (NM_005309) Human Recombinant Protein

Product Specifications

Background

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1) ; also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]

Overview

Recombinant protein of human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT)

Synonyms

AAT1; ALT1; GPT1

Gene ID

2875

UniProt

P24298

Accession Number mRNA

NM_005309

Chromosomal Location

8q24.3

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Alanine, aspartate and glutamate metabolism, Metabolic pathways

Field of Research

Alanine, aspartate and glutamate metabolism, Metabolic pathways

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome

Molecular Weight

54.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.