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MRPL32 (NM_031903) Human Recombinant Protein

Product Specifications

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq, Jul 2008]

Overview

Recombinant protein of human mitochondrial ribosomal protein L32 (MRPL32), nuclear gene encoding mitochondrial protein

Synonyms

BMRP-59b; HSPC283; L32mt; MRP-L32

Gene ID

64983

UniProt

Q9BYC8, A4D1V4

Accession Number mRNA

NM_031903

Chromosomal Location

7p14.1

Expression System

HEK293T

Tag

C-Myc/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Molecular Weight

21.2 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

MRPL32

Species

Human

Protein ID

NP_114109

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/64983

Uniprot URL

https://www.uniprot.org/uniprot/Q9BYC8

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_031903

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_114109

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