Vinculin (VCL) (NM_003373) Human Recombinant Protein

Product Specifications

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Overview

Recombinant protein of human vinculin (VCL), transcript variant 2

Synonyms

CMD1W; CMH15; HEL114; MV; MVCL

Gene ID

7414

UniProt

P18206, A0A024QZN4, B3KXA2

Accession Number mRNA

NM_003373

Chromosomal Location

10q22.2

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Adherens junction, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton

Field of Research

Adherens junction, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Activity

26439954

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome

Molecular Weight

116.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.