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Osteopontin (SPP1) (NM_001040058) Human Recombinant Protein

Product Specifications

Background

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Overview

Purified recombinant protein of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 1

Synonyms

BNSP; BSPI; ETA-1; OPN

Gene ID

6696

UniProt

P10451, A0A024RDE2

Accession Number mRNA

NM_001040058

Chromosomal Location

4q22.1

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway

Field of Research

ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Activity

29959420

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome, Secreted Protein

Molecular Weight

33.7 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

SPP1

Species

Human

Protein ID

NP_001035147

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/6696

Uniprot URL

https://www.uniprot.org/uniprot/P10451

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_001040058

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_001035147

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