SUCLA2 (NM_003850) Human Recombinant Protein

Product Specifications

Background

Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6. [provided by RefSeq, Jul 2008]

Overview

Recombinant protein of human succinate-CoA ligase, ADP-forming, beta subunit (SUCLA2), nuclear gene encoding mitochondrial protein

Synonyms

A-BETA; A-SCS; LINC00444; MTDPS5; SCS-betaA

Gene ID

8803

UniProt

Q9P2R7, E5KS60, Q9Y4T0

Accession Number mRNA

NM_003850

Chromosomal Location

13q14.2

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Citrate cycle (TCA cycle), Metabolic pathways, Propanoate metabolism

Field of Research

Citrate cycle (TCA cycle), Metabolic pathways, Propanoate metabolism

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Molecular Weight

44.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.