ME1 (NM_002395) Human Recombinant Protein

Product Specifications

Background

This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008]

Overview

Recombinant protein of human malic enzyme 1, NADP (+) -dependent, cytosolic (ME1)

Synonyms

HUMNDME; MES

Gene ID

4199

UniProt

P48163

Accession Number mRNA

NM_002395

Chromosomal Location

6q14.2

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Metabolic pathways, PPAR signaling pathway, Pyruvate metabolism

Field of Research

Metabolic pathways, PPAR signaling pathway, Pyruvate metabolism

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Molecular Weight

64 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.