GCLM (NM_002061) Human Recombinant Protein

Product Specifications

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Overview

Recombinant protein of human glutamate-cysteine ligase, modifier subunit (GCLM)

Synonyms

GLCLR

Gene ID

2730

UniProt

P48507

Accession Number mRNA

NM_002061

Chromosomal Location

1p22.1

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Glutathione metabolism, Metabolic pathways

Field of Research

Glutathione metabolism, Metabolic pathways

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome

Molecular Weight

30.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.