MRCL3 (MYL12A) (NM_006471) Human Recombinant Protein

Product Specifications

Background

This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF) /Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.[provided by RefSeq, Dec 2014]

Overview

Recombinant protein of human myosin, light chain 12A, regulatory, non-sarcomeric (MYL12A)

Synonyms

HEL-S-24; MLC-2B; MLCB; MRCL3; MRLC3; MYL2B

Gene ID

10627

UniProt

P19105

Accession Number mRNA

NM_006471

Chromosomal Location

18p11.31

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction

Field of Research

Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Molecular Weight

19.6 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.