GCLC (NM_001498) Human Recombinant Protein
Product Specifications
Background
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]
Synonyms
GCL; GCS; GLCL; GLCLC
Gene ID
2729
UniProt
P48506, Q14TF0
Accession Number mRNA
NM_001498
Chromosomal Location
6p12.1
Expression System
HEK293T
Tag
C-Myc/DDK
Related Pathways
Glutathione metabolism, Metabolic pathways
Field of Research
Glutathione metabolism, Metabolic pathways
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Function
Druggable Genome
Molecular Weight
72.6 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
GCLC
Species
Human
Protein ID
NP_001489
Overview
Recombinant protein of human glutamate-cysteine ligase, catalytic subunit (GCLC)
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/2729
Uniprot URL
https://www.uniprot.org/uniprot/P48506
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_001498
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_001489
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
