GCLC (NM_001498) Human Recombinant Protein

Product Specifications

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]

Overview

Recombinant protein of human glutamate-cysteine ligase, catalytic subunit (GCLC)

Synonyms

GCL; GCS; GLCL; GLCLC

Gene ID

2729

UniProt

P48506, Q14TF0

Accession Number mRNA

NM_001498

Chromosomal Location

6p12.1

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Glutathione metabolism, Metabolic pathways

Field of Research

Glutathione metabolism, Metabolic pathways

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome

Molecular Weight

72.6 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.