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DDX4 (NM_024415) Human Recombinant Protein

Product Specifications

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Overview

Recombinant protein of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 1

Synonyms

VASA

Gene ID

54514

UniProt

Q9NQI0

Accession Number mRNA

NM_024415

Chromosomal Location

5q11.2

Expression System

HEK293T

Tag

C-Myc/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Molecular Weight

79.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

DDX4

Species

Human

Protein ID

NP_077726

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/54514

Uniprot URL

https://www.uniprot.org/uniprot/Q9NQI0

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_024415

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_077726

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