BRF1 (NM_001519) Human Recombinant Protein
Product Specifications
Background
This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011]
Synonyms
BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p; TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90
Gene ID
2972
UniProt
Q92994
Accession Number mRNA
NM_001519
Chromosomal Location
14q32.33
Expression System
HEK293T
Tag
C-Myc/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Function
Transcription Factors
Molecular Weight
73.7 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
BRF1
Species
Human
Protein ID
NP_001510
Overview
Recombinant protein of human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 1
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/2972
Uniprot URL
https://www.uniprot.org/uniprot/Q92994
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_001519
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_001510
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