CCBL1 (KYAT1) (NM_004059) Human Recombinant Protein
Product Specifications
Background
This gene encodes a cytosolic enzyme that is responsible for the metabolism of cysteine conjugates of certain halogenated alkenes and alkanes. This metabolism can form reactive metabolites leading to nephrotoxicity and neurotoxicity. Increased levels of this enzyme have been linked to schizophrenia. Multiple transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Synonyms
CCBL1; GTK; KAT1; KATI
Gene ID
883
UniProt
Q16773, A8K563
Accession Number mRNA
NM_004059
Chromosomal Location
9q34.11
Expression System
HEK293T
Tag
C-Myc/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Activity
The specific activity of KATI was determined by measuring the product Kynurenic acid formation from a conversion of Kynurenine. The reaction was carried out at 37? for 15min in the buffer containing 100 mM PBS, pH7.4, 2 mM a-oxoglutarate, 40µM PLP (pyridoxal 5'-phosphate), and 0.5 mM kynurenine as the substrate.
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Molecular Weight
47.7 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
KYAT1
Species
Human
Protein ID
NP_004050
Overview
Recombinant protein of human cysteine conjugate-beta lyase, cytoplasmic (CCBL1), transcript variant 1
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/883
Uniprot URL
https://www.uniprot.org/uniprot/Q16773
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_004059
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_004050
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