P15 INK4b (CDKN2B) (NM_078487) Human Recombinant Protein

Product Specifications

Background

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]

Overview

Recombinant protein of human cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 2

Synonyms

CDK4I; INK4B; MTS2; P15; p15INK4b; TP15

Gene ID

1030

UniProt

P42772

Accession Number mRNA

NM_078487

Chromosomal Location

9p21.3

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Cell cycle, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway

Field of Research

Cell cycle, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome

Molecular Weight

7.9 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.