LCK (NM_005356) Human Recombinant Protein

Product Specifications

Background

This gene is a member of the Src family of protein tyrosine kinases (PTKs) . The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Aug 2016]

Overview

Recombinant protein of human lymphocyte-specific protein tyrosine kinase (LCK), transcript variant 2

Synonyms

IMD22; LSK; p56lck; pp58lck; YT16

Gene ID

3932

UniProt

P06239, A0A0S2Z3Y8

Accession Number mRNA

NM_005356

Chromosomal Location

1p35.2

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Natural killer cell mediated cytotoxicity, Primary immunodeficiency, T cell receptor signaling pathway

Field of Research

Natural killer cell mediated cytotoxicity, Primary immunodeficiency, T cell receptor signaling pathway

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome, Protein Kinase, Stem cell - Pluripotency

Molecular Weight

57.8 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.