NFE4 (NM_001085386) Human Recombinant Protein
Product Specifications
Background
The erythroid-specific protein encoded by this gene, and the ubiquitous transcription factor CP2, form the stage selector protein (SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform (22 kDa) acts as an activator, the short isoform (14 kDa) has been shown to repress gamma-globin gene expression. This gene is located in an intron of the FBXL13 gene on the opposite strand. [provided by RefSeq, Jul 2008]
Synonyms
NF-E4
Gene ID
58160
Accession Number mRNA
NM_001085386
Chromosomal Location
7q22.1
Expression System
HEK293T
Tag
C-Myc/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Molecular Weight
18.9 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
NFE4
Species
Human
Protein ID
NP_001078855
Overview
Recombinant protein of human transcription factor NF-E4 (NF-E4)
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/58160
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_001085386
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_001078855
Curated Selection
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