Smek1 (PPP4R3A) (NM_032560) Human Recombinant Protein

Product Specifications

Background

Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.[UniProtKB/Swiss-Prot Function]

Overview

Recombinant protein of human SMEK homolog 1, suppressor of mek1 (Dictyostelium) (SMEK1)

Synonyms

FLFL1; KIAA2010; MSTP033; PP4R3A; smk-1; smk1

Gene ID

55671

UniProt

Q6IN85

Accession Number mRNA

NM_032560

Chromosomal Location

14q32.12

Expression System

HEK293T

Tag

C-Myc/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Molecular Weight

93.7 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.