USP37 (NM_020935) Human Recombinant Protein

Product Specifications

Background

Deubiquitinase that antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Also mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains in vitro. Phosphorylation at Ser-628 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2) (PubMed:21596315) . Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1 (PubMed:27296872) .[UniProtKB/Swiss-Prot Function]

Overview

Recombinant protein of human ubiquitin specific peptidase 37 (USP37)

Synonyms

KIAA1594; MGC117261

Gene ID

57695

UniProt

Q86T82, A0A024R416

Accession Number mRNA

NM_020935

Chromosomal Location

2q35

Expression System

HEK293T

Tag

C-Myc/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Protease

Molecular Weight

110 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.