PUF60 (NM_078480) Human Recombinant Protein

Product Specifications

Background

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Overview

Recombinant protein of human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1

Synonyms

FIR; RoBPI; SIAHBP1; VRJS

Gene ID

22827

UniProt

Q9UHX1

Accession Number mRNA

NM_078480

Chromosomal Location

8q24.3

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Spliceosome

Field of Research

Spliceosome

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Activity

2625309529541951

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Molecular Weight

59.7 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.