Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

DNMT1 (NM_001130823) Human Recombinant Protein

Product Specifications

Background

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Synonyms

ADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT

Gene ID

1786

UniProt

P26358, I6L9H2

Accession Number mRNA

NM_001130823

Chromosomal Location

19p13.2

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Cysteine and methionine metabolism, Metabolic pathways

Field of Research

Cysteine and methionine metabolism, Metabolic pathways

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Activity

DNMT1 activity verified in a biochemical assay: DNMT1 (DNA (cytosine-5-) -methyltransferase 1) (PH33440M5) is a key methyltransferase that is responsible for maintaining methylation patterns established in development. DNMT1 preferentially methylates hemi-methylated CpG di-nucleotides and associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand. Varying concentrations of DNMT1 were added to a microplate containing a bound methyltransferase substrate. After incubation, the resulting methylated DNA residues were detected immunologically and a colorimetric signal was generated and measured.

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome, Transcription Factors

Molecular Weight

184.6 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

DNMT1

Species

Human

Protein ID

NP_001124295

Overview

Recombinant protein of human DNA (cytosine-5-) -methyltransferase 1 (DNMT1), transcript variant 1

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/1786

Uniprot URL

https://www.uniprot.org/uniprot/P26358

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_001130823

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_001124295

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Sheep Cytoplasmic dynein 1 light intermediate chain 1 (DYNC1LI1) ELISA Kit
E14C2277-01 48 Well

Sheep Cytoplasmic dynein 1 light intermediate chain 1 (DYNC1LI1) ELISA Kit

Ask
View Details
Sheep Cytoplasmic dynein 1 light intermediate chain 1 (DYNC1LI1) ELISA Kit
E14C2277-02 96 Well

Sheep Cytoplasmic dynein 1 light intermediate chain 1 (DYNC1LI1) ELISA Kit

Ask
View Details
trans-3'-Hydroxy Cotinine Benzoate
sc-490278 10 mg

trans-3'-Hydroxy Cotinine Benzoate

Ask
View Details
2,2-Difluoro-1-iodopropane
PC1003256-01 100 mg

2,2-Difluoro-1-iodopropane

Ask
View Details
2,2-Difluoro-1-iodopropane
PC1003256-02 250 mg

2,2-Difluoro-1-iodopropane

Ask
View Details
2,2-Difluoro-1-iodopropane
PC1003256-03 1 g

2,2-Difluoro-1-iodopropane

Ask
View Details