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MYL12B (NM_001144946) Human Recombinant Protein

Product Specifications

Background

The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]) .[supplied by OMIM, Mar 2008]

Synonyms

MLC-B; MRLC2

Gene ID

103910

UniProt

O14950

Accession Number mRNA

NM_001144946

Chromosomal Location

18p11.31

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction

Field of Research

Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Molecular Weight

17.6 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

MYL12B

Species

Human

Protein ID

NP_001138418

Overview

Purified recombinant protein of Homo sapiens myosin, light chain 12B, regulatory (MYL12B), transcript variant 4

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/103910

Uniprot URL

https://www.uniprot.org/uniprot/O14950

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_001144946

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_001138418

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