Cardiac Troponin T (TNNT2) (NM_000364) Human Recombinant Protein

Product Specifications

Background

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

Overview

Purified recombinant protein of Human troponin T type 2 (cardiac) (TNNT2), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50 ug

Synonyms

CMD1D; CMH2; CMPD2; cTnT; LVNC6; RCM3; TnTC

Gene ID

7139

UniProt

P45379

Accession Number mRNA

NM_000364

Chromosomal Location

1q32.1

Expression System

E. coli

Tag

N-HIS

Related Pathways

Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)

Field of Research

Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)

Concentration

>50 ug/mL as determined by spectrophotometrically (A280) method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Lyophilized

Buffer

Lyophilized from 0.01 M HCl, reconstitute with Tris/urea buffer (20 mM Tris pH7.5, 7 M urea, 5 mM EDTA, 15 mM β-mercaptoethanol) .

Function

Druggable Genome

Molecular Weight

35.4KDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.