MSH2 (NM_000251) Human Recombinant Protein

Product Specifications

Background

This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) . When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Overview

Purified recombinant protein of Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2), Tyr121-Ile169-GGGGS-Pro837-Lys907, with N-terminal His tag, expressed in E.coli, 50ug

Synonyms

COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2; MMRCS2

Gene ID

4436

UniProt

P43246

Accession Number mRNA

NM_000251

Chromosomal Location

2p21-p16.3

Expression System

E. coli

Tag

N-His

Related Pathways

Colorectal cancer, Mismatch repair, Pathways in cancer

Field of Research

Colorectal cancer, Mismatch repair, Pathways in cancer

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150mM NaCl, 10% glycerol

Function

Druggable Genome, Stem cell - Pluripotency

Molecular Weight

13.7 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.