CD22 (NM_001771) Human Recombinant Protein

Product Specifications

Background

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Human CD22 molecule (CD22), transcript variant 1, Asp20-Ala330, with N-terminal His tag, expressed in E.coli, 50ug

Synonyms

SIGLEC-2; SIGLEC2

Gene ID

933

UniProt

P20273, Q0EAF5

Accession Number mRNA

NM_001771

Chromosomal Location

19q13.12

Expression System

E. coli

Tag

N-His

Related Pathways

B cell receptor signaling pathway, Cell adhesion molecules (CAMs), Hematopoietic cell lineage

Field of Research

B cell receptor signaling pathway, Cell adhesion molecules (CAMs), Hematopoietic cell lineage

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

>80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

50mM Tris, pH8.0, 8M Urea

Function

Druggable Genome, Transmembrane

Molecular Weight

35.4 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.