CAS9-D10A Streptococcus Pyogenes Recombinant Protein

Product Specifications

Overview

Purified recombinant protein of mutant (D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli

Expression System

E. coli

Tag

N-His

Concentration

>50 ug/mL as determined by Bradford protein assay,10 µM is equal to 1.61 mg/ml.

Endotoxin

< 0.5 EU per 1 µg of the protein by the LAL

Purity

> 95% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

10mM Tris, 300mM NaCl, 0.1mM EDTA, 1mM DTT, pH7.4, 50% glycerol.

Molecular Weight

165.0 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.