Urm1 (NM_026615) Mouse Recombinant Protein

Product Specifications

Background

Acts as a sulfur carrier required for 2-thiolation of mcm (5) S (2) U at tRNA wobble positions of cytosolic tRNA (Lys), tRNA (Glu) and tRNA (Gln) . Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm (5) S (2) U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse ubiquitin related modifier 1 (Urm1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

2900073H19Rik

Gene ID

68205

UniProt

Q9D2P4

Accession Number mRNA

NM_026615

Chromosomal Location

2 B

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

11.3 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.