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Polr2f (NM_027231) Mouse Recombinant Protein

Selected Variant

PM48950M5

Size: 20 µg

Product Specifications

Background

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse polymerase (RNA) II (DNA directed) polypeptide F (Polr2f), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

1810060D16Rik; RPB6

Gene ID

69833

UniProt

P61219

Accession Number mRNA

NM_027231

Chromosomal Location

15 37.7 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

14.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

Polr2f

Species

Mouse

Protein ID

NP_081507

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/69833

Uniprot URL

https://www.uniprot.org/uniprot/P61219

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_027231

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_081507

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