Polr2f (NM_027231) Mouse Recombinant Protein
Selected Variant
PM48950M5
Size: 20 µg
Product Specifications
Background
DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity) .[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse polymerase (RNA) II (DNA directed) polypeptide F (Polr2f), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
1810060D16Rik; RPB6
Gene ID
69833
UniProt
P61219
Accession Number mRNA
NM_027231
Chromosomal Location
15 37.7 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
14.5 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Polr2f
Species
Mouse
Protein ID
NP_081507
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/69833
Uniprot URL
https://www.uniprot.org/uniprot/P61219
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_027231
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_081507
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