Rfk (NM_019437) Mouse Recombinant Protein

Product Specifications

Background

Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse riboflavin kinase (Rfk), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

0610038L10Rik; AF031381; KOI-4

Gene ID

54391

UniProt

Q8CFV9

Accession Number mRNA

NM_019437

Chromosomal Location

19 B

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

17.9 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.