Rfk (NM_019437) Mouse Recombinant Protein
Selected Variant
PM48752M5
Size: 20 µg
Product Specifications
Background
Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase (By similarity) .[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse riboflavin kinase (Rfk), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
0610038L10Rik; AF031381; KOI-4
Gene ID
54391
UniProt
Q8CFV9
Accession Number mRNA
NM_019437
Chromosomal Location
19 B
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
17.9 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Rfk
Species
Mouse
Protein ID
NP_062310
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/54391
Uniprot URL
https://www.uniprot.org/uniprot/Q8CFV9
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_019437
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_062310
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