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Atp5h (NM_027862) Mouse Recombinant Protein

Product Specifications

Background

Mitochondrial membrane ATP synthase (F (1) F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F (1) - containing the extramembraneous catalytic core, and F (0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F (1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F (0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha (3) beta (3) subcomplex and subunit a/ATP6 static relative to the rotary elements.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse ATP synthase, H+ transporting, mitochondrial F0 complex, subunit D (Atp5h), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

0610009D10Rik

Gene ID

71679

UniProt

Q9DCX2

Accession Number mRNA

NM_027862

Chromosomal Location

11 E2

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

18.7 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

Atp5h

Species

Mouse

Protein ID

NP_082138

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/71679

Uniprot URL

https://www.uniprot.org/uniprot/Q9DCX2

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_027862

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_082138

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