Ube2f (NM_026454) Mouse Recombinant Protein

Product Specifications

Background

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse ubiquitin-conjugating enzyme E2F (putative) (Ube2f), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

2510010F15Rik; AI851109

Gene ID

67921

UniProt

Q9CY34

Accession Number mRNA

NM_026454

Chromosomal Location

1 D

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

21.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.