Ube2f (NM_026454) Mouse Recombinant Protein
Product Specifications
Background
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse ubiquitin-conjugating enzyme E2F (putative) (Ube2f), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
2510010F15Rik; AI851109
Gene ID
67921
UniProt
Q9CY34
Accession Number mRNA
NM_026454
Chromosomal Location
1 D
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
21.1 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Ube2f
Species
Mouse
Protein ID
NP_080730
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/67921
Uniprot URL
https://www.uniprot.org/uniprot/Q9CY34
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_026454
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_080730
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